WNV VLPs pVRC8400

LOCUS       WNV VLPs pVRC8400                7571 bp ds-DNA     circular SYN 04-APR-2024
DEFINITION  West Nile Virus prM-E structural cassette (NY99 sequence) in 
            pVRC8400.
ACCESSION   .
VERSION     .
KEYWORDS    WNV VLPs pVRC8400
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Chao LH, Klein DE, Schmidt AG, Pena JM, Harrison SC
  TITLE     Sequential conformational rearrangements in flavivirus membrane 
            fusion.
  JOURNAL   Elife. 2014 Dec 5;4. doi: 10.7554/eLife.04389.
  PUBMED    25479384
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 4, 2024 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3123..3567
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     protein_bind    complement(3594..3614)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3626..4225
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3626..3645)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4122..4142
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4239..4463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4830..4847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5001..5589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5081..5100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6131..6946)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6854..6873
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6938..6957
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     7224..7242
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7280..7302)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7402..7421
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctcca tcggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
      721 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
      781 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
      841 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
      901 gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc gcgccaccag
      961 acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct gcagtcaccg
     1021 tcgtcgacca ccatggatgc aatgaagaga gggctctgct gtgtgctgct gctgtgtgga
     1081 gcagtcttcg tttcgcccag cgctagcgtt accctctcta acttccaagg gaaggtgatg
     1141 atgacggtaa atgctactga cgtcacagat gtcatcacga ttccaacagc tgctggaaag
     1201 aacctatgca ttgtcagagc aatggatgtg ggatacatgt gcgatgatac tatcacttat
     1261 gaatgcccag tgctgtcggc tggtaatgat ccagaagaca tcgactgttg gtgcacaaag
     1321 tctgcagtct acgtcaggta tggaagatgc accaagacgc gtcactcaag acgcagtcgg
     1381 aggtcactga cagtgcagac acacggtgaa agcactctag cgaacaagaa gggggcttgg
     1441 atggacagca ccaaggccac aaggtatttg gtaaaaacag aatcatggat cttgaggaac
     1501 cctggatatg ccctggtggc agccgtcatt ggttggatgc ttgggagcaa caccatgcag
     1561 agagttgtgt ttgtcgtgct attgcttttg gtggccccag cttacagctt caactgcctt
     1621 ggaatgagca acagagactt cttggaagga gtgtctggag caacatgggt agatctggtt
     1681 ctcgaaggcg acagctgcgt gactatcatg tctaaggaca agcctaccat cgatgtgaag
     1741 atgatgaata tggaggcggc caacctggca gaggtccgca gttattgcta tttggctacc
     1801 gtcagcgatc tctccaccaa agctgcgtgc ccgacgatgg gagaagctca caatgacaaa
     1861 cgtgctgacc cagcttttgt gtgcagacaa ggagtggtgg acaggggctg gggcaacggc
     1921 tgcggactat ttggcaaagg aagcattgac acatgcgcca aatttgcctg ctctaccaag
     1981 gcaataggaa gaaccatctt gaaagagaat atcaagtacg aagtggccat ttttgtccat
     2041 ggaccaacta ctgtggagtc gcacggaaac tactccacac aggttggagc cactcaggcc
     2101 ggccgattca gcatcactcc tgcggcgcct tcatacacac taaaactcgg agaatatgga
     2161 gaggtgacag tggactgtga accacggtca gggattgaca ccaatgcata ctacgtgatg
     2221 actgttggaa caaagacgtt cttggtccat cgtgagtggt tcatggacct caacctccct
     2281 tggagcagtg ctggaagtac tgtgtggagg aacagagaga cgttaatgga gtttgaggaa
     2341 ccacacgcca cgaagcagtc tgtgatagca ttgggctcac aagagggagc tctgcatcag
     2401 gcattggctg gagccattcc tgtggaattt tcaagcaaca ctgtcaagtt gacgtcgggt
     2461 catttgaagt gtagagtgaa gatggaaaaa ttgcagttga agggaacaac ctatggcgtc
     2521 tgttcaaagg ctttcaagtt tcttgggact cccgcagaca caggtcacgg cactgtggtg
     2581 ttggaattgc agtacactgg cacggatgga ccttgcaaag ttcctatctc gtcagtggct
     2641 tcattgaacg acctaacgcc agtgggcaga ctagtcactg tcaacccttt tgtttcagtg
     2701 gccacggcca acgctaaggt cctgattgaa ttggaaccac cctttggaga ctcatacata
     2761 gtggtgggca gaggagaaca acagatcaat caccattggc acaagtctgg aagcagcatt
     2821 ggcaaagcct ttacaaccac cctcaaagga gcgcagagac tagccgctct aggagacaca
     2881 gcttgggact ttggatcagt tggaggggtg ttcacctcag ttgggaaggc tgtccatcaa
     2941 gtgttcggag gagcattccg ctcactgttc ggaggcatgt cctggataac gcaaggattg
     3001 ctgggggctc tcctgttgtg gatgggcatc aatgctcgtg ataggtccat agctctcacg
     3061 tttctcgcag ttggaggagt tctgctcttc ctctccgtga acgtgcacgc ttgagcggcc
     3121 gcagcaggtt tccccaatga cacaaaacgt gcaacttgaa actccgcctg gtctttccag
     3181 gtctagaggg gtaacacttt gtactgcgtt tggctccacg ctcgatccac tggcgagtgt
     3241 tagtaacagc actgttgctt cgtagcggag catgacggcc gtgggaactc ctccttggta
     3301 acaaggaccc acggggccaa aagccacgcc cacacgggcc cgtcatgtgt gcaaccccag
     3361 cacggcgact ttactgcgaa acccacttta aagtgacatt gaaactggta cccacacact
     3421 ggtgacaggc taaggatgcc cttcaggtac cccgaggtaa cacgcgacac tcgggatctg
     3481 agaaggggac tggggcttct ataaaagcgc tcggtttaaa aagcttctat gcctgaatag
     3541 gtgaccggag gtcggcacct ttcctttgca attactgacc ctatgataca ctgcaacttt
     3601 attatacaaa gttggaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     3661 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3721 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3781 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3841 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3901 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3961 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4021 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4081 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4141 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4201 catgacccgc aagcccggtg cctgaggatc cagatctgct gtgccttcta gttgccagcc
     4261 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4321 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4381 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4441 tggggatgcg gtgggctcta tgggtaccca ggtgctgaag aattgacccg gttcctcctg
     4501 ggccagaaag aagcaggcac atccccttct ctgtgacaca ccctgtccac gcccctggtt
     4561 cttagttcca gccccactca taggacactc atagctcagg agggctccgc cttcaatccc
     4621 acccgctaaa gtacttggag cggtctctcc ctccctcatc agcccaccaa accaaaccta
     4681 gcctccaaga gtgggaagaa attaaagcaa gataggctat taagtgcaga gggagagaaa
     4741 atgcctccaa catgtgagga agtaatgaga gaaatcatag aattttaagg ccatgattta
     4801 aggccatcat ggccttaatc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4861 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4921 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4981 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5041 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5101 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5161 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5221 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5281 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5341 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5401 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     5461 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5521 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5581 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5641 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5701 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5761 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5821 gttgcctgac tcgggggggg ggggcgctga ggtctgcctc gtgaagaagg tgttgctgac
     5881 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     5941 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     6001 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt
     6061 caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac aaccaattaa
     6121 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     6181 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     6241 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     6301 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     6361 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
     6421 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     6481 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     6541 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     6601 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     6661 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     6721 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     6781 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     6841 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     6901 ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     6961 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     7021 tgtaacatca gagattttga gacacaacgt ggctttcccc ccccccccat tattgaagca
     7081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7201 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     7261 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7321 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7381 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7441 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcagattg gctattggcc
     7501 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     7561 accgccatgt t
//